calibexternal.massvector {mscalib}R Documentation

External Calbiration

Description

Perfroms external calibration. Obtains the error model by calling getextcalib and corrects the masses in the massvector for the errror.

Usage

## S3 method for class 'massvector':
calibexternal(object,ppg,calib,...)

Arguments

... further parameters.
calib a massvector with exact masses of the calibration sample (ppg).
object massvector
ppg either a massvector or massvectorlist with masses of the calibration sample (e.g. poly-(propylene glycol)).

Details

In case of external calibration some sample spots are only dedicated to calibration. Calibration samples which produces equidistant peaks, which exact masses are known, can be used to precisely estimate the mass dependent error function.

Value

massvector calibrated massvector

Author(s)

Witold Wolski wolski@molgen.mpg.de

References

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Gobom J, Mueller M, Egelhofer V, Theiss D, Lehrach H, Nordhoff E, 2002. A calibration method that simplifies and improves accurate determination of peptide molecular masses by MALDI-TOF MS.Anal Chem. 74(15):3915-23.
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Wolski http://www.molgen.mpg.de/~wolski/mscalib

See Also

applyextcalib.massvectorlist, getextcalib.massvector, getextcalib.massvectorlist, calibexternal.massvector, applycalib.calibspline,

Examples

 data(ppg)
 data(mv1)
 mv2<-calibexternal(mv1,ppg)
 compare(mv1,mv2)

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