S27 {qpcR} | R Documentation |
The data was obtained from cDNA reverse transcribed from the isolated RNA of 15 different testicular biopsies.
data(S27)
A data frame with cycle number and 15 different qPCR runs (F1 - F15).
The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).
Andrej-Nikolai Spiess & Caroline Feig, Department of Andrology, University Hospital Hamburg, Germany.
data(S27) ### on single data m1 <- multdrc(F1 ~ Cycles, data = S27, fct = l5()) pcrplot(m1) ### defining 15 replicates repData <- repform(S27[,1:16], c(0, rep(1, 15))) m2 <- multdrc(values ~ Cycles, curve = Curve, data = repData, fct = l5()) pcrplot(m2, type = "all")