inVitro {CalciOMatic}R Documentation

Calcium Transients and Fura 2 Loading Curves

Description

Data from 20 in vitro experiments performed on cockroaches (Periplaneta americana) antennal lobe neurons. Each data set contains a list of experimental results made of the fura 2 loading curve (measured at 360 nm) and 3 (or 2) calcium transients (measured at 340 and 380 nm).

Usage

data(inVitro)

Format

The data are stored as integer. inVitro contains 20 sublists. Each sublist corresponds to one experiment and contains:
time360 A vector of times (in s) at which fluorescence measurements
at the isosbestic wavelength (360 nm) were taken
adu360 A vector of fluorescence measurements at 360 nm
adu360Background Background fluorescence measurements at 360 nm
P360 Number of on-chip binned pixels used for the measurements at 360 nm
P360Background Number of on-chip binned pixels used for the measurements of background
fluorescence at 360 nm. If different from P360 then the pixels used for
background measurement were different than the one used for
loading curve measurements
onChipBinning360 Number of physical pixels binned on-chip for the 360 nm measurements
exposureTime360 Exposure time used at 360 nm (in s)
adu340Background Background fluorescence measurements at 340 nm
adu380Background Background fluorescence measurements at 380 nm
furaPipette The total fura concentration in the pipette (in microM)
exposureTime340 Exposure time used at 340 nm (in s)
exposureTime380 Exposure time used at 380 nm (in s)
P Number of on-chip binned pixels
PBackground Number of on-chip binned pixels used for the measurements of background
fluorescence at 340 and 380 nm. If different from P then the pixels used for
background measurement were different than the one used for
transient measurements
onChipBinning Number of physical pixels binned on-chip for the 340 and 380 nm measurements
stim1, stim2 and stim3 List with calcium transient data (see below)

Each element stim1, stim2 and when a third transient was measured, stim3, are lists with the following components:
time A vector of times (in s) at which fluorescence measurements were taken
adu340 A vector of fluorescence measurements at 340 nm
adu380 A vector of fluorescence measurements at 380 nm

Details

Break-in time is 0.

inVitro contains data recorded in vitro (from cultured cells) from antennal lobe neurons for a total of 20 experiments. Experiments 5 and 16 in the inVitro data set have only 2 transients instead of 3.

Source

Andreas Pippow andreas.pippow@uni-koeln.de did the experiments

Examples

data(inVitro)

[Package CalciOMatic version 1.1-3 Index]