inVitro {CalciOMatic} | R Documentation |
Data from 20 in vitro experiments performed on cockroaches (Periplaneta americana) antennal lobe neurons. Each data set contains a list of experimental results made of the fura 2 loading curve (measured at 360 nm) and 3 (or 2) calcium transients (measured at 340 and 380 nm).
data(inVitro)
The data are stored as integer
. inVitro
contains 20
sublists. Each sublist corresponds to one experiment and contains:
time360 | A vector of times (in s) at which fluorescence measurements |
at the isosbestic wavelength (360 nm) were taken | |
adu360 | A vector of fluorescence measurements at 360 nm |
adu360Background | Background fluorescence measurements at 360 nm |
P360 | Number of on-chip binned pixels used for the measurements at 360 nm |
P360Background | Number of on-chip binned pixels used for the measurements of background |
fluorescence at 360 nm. If different from P360 then the pixels used for |
|
background measurement were different than the one used for | |
loading curve measurements | |
onChipBinning360 | Number of physical pixels binned on-chip for the 360 nm measurements |
exposureTime360 | Exposure time used at 360 nm (in s) |
adu340Background | Background fluorescence measurements at 340 nm |
adu380Background | Background fluorescence measurements at 380 nm |
furaPipette | The total fura concentration in the pipette (in microM) |
exposureTime340 | Exposure time used at 340 nm (in s) |
exposureTime380 | Exposure time used at 380 nm (in s) |
P | Number of on-chip binned pixels |
PBackground | Number of on-chip binned pixels used for the measurements of background |
fluorescence at 340 and 380 nm. If different from P then the pixels used for |
|
background measurement were different than the one used for | |
transient measurements | |
onChipBinning | Number of physical pixels binned on-chip for the 340 and 380 nm measurements |
stim1, stim2 and stim3 | List with calcium transient data (see below) |
Each element stim1
, stim2
and when a third transient was
measured, stim3
, are lists with the following components:
time | A vector of times (in s) at which fluorescence measurements were taken |
adu340 | A vector of fluorescence measurements at 340 nm |
adu380 | A vector of fluorescence measurements at 380 nm |
Break-in time is 0.
inVitro
contains data recorded in vitro (from cultured cells)
from antennal lobe neurons for a total of 20 experiments. Experiments
5 and 16 in the inVitro
data set have only 2 transients instead
of 3.
Andreas Pippow andreas.pippow@uni-koeln.de did the experiments
data(inVitro)