batschetal {qpcR} | R Documentation |
High quality 4-fold dilution experiments with 5 dilution steps and 3 replicates each. See 'Details' for the different setups.
batsch1 batsch2 batsch3 batsch4 batsch5
Data frames with the PCR cycles and 15 qPCR runs with 3 replicates of five 4-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).
The real-time PCR was conducted with a Lightcycler 1.0 instrument using the following setups:
batsch1: Primers for rat SLC6A14, Taqman probes
batsch2: Primers for human SLC22A13, Taqman probes
batsch3: Primers for pig EMT, Taqman probes
batsch4: Primers for chicken ETT, SybrGreen
batsch5: Primers for human GAPDH, SybrGreen
Additional File 5 to the paper.
Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence.
Batsch A et al., BMC Bioinformatics, 2008, 9: 95.
data(batsch1) ml <- modlist(batsch1, model = l4) plot(ml)