eff {qpcR}R Documentation

The amplification efficiency curve of a fitted object

Description

Calculates the efficiency curve from the fitted object by E = frac{F(n)}{F(n-1)}, with E = efficiency, F = raw fluorescence, n = Cycle number.

Usage

eff(object, sequence = NULL, plot = FALSE)

Arguments

object an object of class 'pcrfit'.
sequence a 3-element vector (from; to; by) defining the sequence for the efficiency curve, defaults to [min(Cycles), max(Cycles)] with 100 points per cycle.
plot should the efficiency be plotted?

Value

A list with the following components:

eff.x the cycle points.
eff.y the efficiency values at eff.x.
effmax.x the cycle number with the highest efficiency.
effmax.y the maximum efficiency.

Author(s)

Andrej-Nikolai Spiess

Examples

m <- pcrfit(reps, 1, 2, l5)

## with default 100 points per cycle
eff(m, plot = TRUE) 

## not all data and only 10 points per cycle
eff(m, sequence = c(5, 35, 0.1), plot = TRUE) 

[Package qpcR version 1.2-4 Index]