eff {qpcR} | R Documentation |
Calculates the efficiency curve from the fitted object by E = frac{F(n)}{F(n-1)}, with E = efficiency, F = raw fluorescence, n = Cycle number.
eff(object, sequence = NULL, plot = FALSE)
object |
an object of class 'pcrfit'. |
sequence |
a 3-element vector (from; to; by) defining the sequence for the efficiency curve, defaults to [min(Cycles), max(Cycles)] with 100 points per cycle. |
plot |
should the efficiency be plotted? |
A list with the following components:
eff.x |
the cycle points. |
eff.y |
the efficiency values at eff.x . |
effmax.x |
the cycle number with the highest efficiency. |
effmax.y |
the maximum efficiency. |
Andrej-Nikolai Spiess
m <- pcrfit(reps, 1, 2, l5) ## with default 100 points per cycle eff(m, plot = TRUE) ## not all data and only 10 points per cycle eff(m, sequence = c(5, 35, 0.1), plot = TRUE)