calibexternal.massvectorlist {mscalib}R Documentation

External Calbiration

Description

Perfroms external calibration of massvectorlist. Obtains the model of the measurment error by getextcalib and corrects the masses for this error.

Usage

## S3 method for class 'massvectorlist':
calibexternal(object,ppg,calib,error=300,...)

Arguments

... further parameters.
calib a massvector with exact masses for the massvectors of the calibration samples (ppg).
error relative error of the measurment in (ppm).
object massvectorlist
ppg either a massvector or massvectorlist with masses of the calibration sample (e.g poly-(propylene glycol) ppg)

Details

In case of external calibration some sample spots are only dedicated to calibration. Calibration samples which produces equidistant peaks, which exact masses are known, can be used to precisely estimate the mass dependent error function.

Value

massvectorlist calibrated massvector

Author(s)

Witold Wolski wolski@molgen.mpg.de

References

-
Gobom J, Mueller M, Egelhofer V, Theiss D, Lehrach H, Nordhoff E, 2002. A calibration method that simplifies and improves accurate determination of peptide molecular masses by MALDI-TOF MS. Anal Chem. 74(15):3915-23.
-
Wolski http://www.molgen.mpg.de/~wolski/mscalib

See Also

calibexternal.massvector, applyextcalib.massvectorlist, getextcalib.massvector, getextcalib.massvectorlist, calibexternal.massvector, applycalib.calibspline,

Examples

 data(mvl)
 data(ppg)
 res <- calibexternal(mvl,ppg,getPPGmasses(),error=150)

[Package mscalib version 0.5.9 Index]