reps3 {qpcR} | R Documentation |
A high quality dilution experiment with six 4-fold dilutions of the cDNA, and three replicates for each dilution.
data(reps3)
A data frame with the PCR cycles and 21 qPCR runs with three replicates of six 4-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).
The real-time PCR was conducted with primers for the S27a housekeeping gene in a MXPro3000P instrument (Stratagene). Data was ROX-normalized, but without smoothing.
Heike Cappallo-Obermann, Bone Marrow Transplantation Unit, University Hospital Hamburg-Eppendorf.
### on single data data(reps3) m1 <- pcrfit(reps3, 1, 2, l4()) pcrplot(m1) ### on replicated data repData <- repform(reps3[,1:4], c(0,1,1,1)) m2 <- drmfit(values ~ Cycles, curve = Curve, data = repData, fct = l5()) pcrplot(m2, type = "all")