rutledge {qpcR} | R Documentation |
A dilution experiment of a 102 bp amplicon with six 10-fold dilutions, with four replicates each in five independent runs.
data(rutledge)
A data frame with the PCR cycles and and the six 10-fold dilutions, with four replicates each and five independent runs, organized as X.Ry.Z, with x = the dilution number, y = the run number and z = the replicate number.
The real-time PCR was conducted for a 102 bp amplicon in an Opticon2 instrument.
Supplemental data 1 to the paper.
Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications. Rutledge RG, Nucleic Acids Research, 2004, 32, e178.
data(rutledge) m1 <- pcrfit(rutledge, 1 , 2, l3()) pcrplot(m1) ### on replicated data repData <- repform(rutledge[,1:5], c(0,1,1,1,1)) m2 <- drmfit(values ~ Cycles, curve = Curve, data = repData, fct = l5()) pcrplot(m2, type = "all")