reps2 {qpcR}R Documentation

Another qPCR dilution experiment with replicates (Roche Lightcycler)

Description

A dilution experiment of two different cDNAs with five 4-fold dilutions of the cDNA, and three replicates for each dilution.

Usage

data(reps2)

Format

A data frame with the PCR cycles and 30 qPCR runs with three replicates of five 4-fold dilutions. The replicates are defined by FX.Y.Z (X = cDNA number, Y = dilution number, Z = replicate number).

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Source

Heike Cappallo-Obermann, Bone Marrow Transplantation Unit, University Hospital Hamburg-Eppendorf.

Examples

### on single data
data(reps2)
m1 <- pcrfit(reps2, 1, 2, l3())
pcrplot(m1)
### on replicated data
repData <- repform(reps2[,1:4], c(0,1,1,1))
m2 <- drmfit(values ~ Cycles, curve = Curve, data = repData, fct = l5())
pcrplot(m2)

[Package qpcR version 1.1-8 Index]