guescini1 {qpcR} | R Documentation |
A high quality 10-fold dilution experiment with 7 dilution steps and 12 replicates each.
data(guescini1)
A data frame with the PCR cycles and 84 qPCR runs with 12 replicates of seven 10-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).
The real-time PCR was conducted with primers for the NADH dehydrogenase 1 in a Lightcycler 480 (Roche). The data is background subtracted.
Supplemental data 1 to the paper.
A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition.
Guescini M et al, BMC Bioinformatics, 2008, 9: 326.
## Not run: data(guescini1) ml <- modlist(guescini1, fct = l5()) pcrplot(ml[[1]]) sapply(2:84, function(x) pcrplot(ml[[x]], add = TRUE)) ## End(Not run)