reps {qpcR} | R Documentation |
A dilution experiment with seven 10-fold dilutions of the cDNA, and four replicates for each dilution.
data(reps)
A data frame with the PCR cycles and 28 qPCR runs with four replicates of seven 10-fold dilutions. The replicates are defined by F1.1 - F1.4 (first dilution), F2.1 - F2.4 (second diltution) etc.
The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).
Andrej-Nikolai Spiess & Nadine Mueller, Institute for Hormone and Fertlity Research, Hamburg, Germany.
data(reps) ### on single data m1 <- pcrfit(reps, 1, 2, l3()) pcrplot(m1) ### on replicated data repData <- repform(reps[,1:5], c(0,1,1,1,1)) m2 <- drmfit(values ~ Cycles, curve = Curve, data = repData, fct = l5()) pcrplot(m2)