eff {qpcR}R Documentation

The amplification efficiency curve of a fitted object

Description

Calculates the efficiency curve from the fitted model by E = frac{F(n)}{F(n-1)}, with E = efficiency, F = raw fluorescence, n = Cycle number.

Usage

  eff(object, sequence = NULL, plot = FALSE)

Arguments

object an object of class 'drc'.
sequence a sequence for the calculated curve, defaults to [min(Cycles), max(Cycles)] with 100 points per cycle.
plot should the efficiency be plotted?

Value

A list with the following components:

eff.x the cycle points.
eff.y the efficiency curve.
effmax the maximum efficiency.

Author(s)

Andrej-Nikolai Spiess

Examples

m <- pcrfit(reps, 1, 2, l5())
eff(m) #with default 100 points per cycle
eff(m, sequence = seq(5, 35, by = 0.1)) #not all data and only 10 points per cycle

[Package qpcR version 1.1-8 Index]