maxRatio {qpcR} | R Documentation |
The maximum ratio (MR) is determined along the interpolated curve of F(x)/F(x-1) and the corresponding cycle number at MR is taken. A measure of qPCR quality (FCN) is then calculated for the curve(s).
maxRatio(ml, plot = TRUE, ...)
ml |
an object of class 'modlist'. |
plot |
Should diagnostic plots be displayed? |
... |
other parameters to be passed to plot . |
In the original paper the authors smooth the datapoints and then apply a cubic spline on the ratio curve, in order to attain a resolution of 0.01 cycles. The function here calculates the ratio along a curve of the sigmoidal fit, which results in essentially the same.
A list with the following components:
mr |
the maximum ratio. |
fcn |
the cycle number at mr . |
fcna |
a corrected fcn , as described in Shain et al. |
names |
the names of the runs as taken from the original dataframe. |
Andrej-Nikolai Spiess
A new method for robust quantitative and qualitative analysis of real-time PCR.
Shain & Clemens, Nucleic Acids Research, 2008, 36, e91.
ml <- modlist(reps, fct = l5()) maxRatio(ml)