S27 {qpcR}R Documentation

PCR data from 15 different testicular biopsies (Roche Lightcycler)

Description

The data was obtained from cDNA reverse transcribed from the isolated RNA of 15 different testicular biopsies.

Usage

data(S27)

Format

A data frame with cycle number and 15 different qPCR runs (F1 - F15).

Details

The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).

Source

Andrej-Nikolai Spiess & Caroline Feig, Department of Andrology, University Hospital Hamburg, Germany.

Examples

data(S27)
m1 <- pcrfit(S27, 1, 2, l5())
pcrplot(m1)     

[Package qpcR version 1.1-8 Index]