guescini1 {qpcR}R Documentation

A qPCR dilution experiment with replicates (Lightcycler 480) from Guescini et al

Description

A high quality 10-fold dilution experiment with 7 dilution steps and 12 replicates each.

Usage

data(guescini1)

Format

A data frame with the PCR cycles and 84 qPCR runs with 12 replicates of seven 10-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).

Details

The real-time PCR was conducted with primers for the NADH dehydrogenase 1 in a Lightcycler 480 (Roche). The data is background subtracted.

Source

Supplemental data 1 to the paper.

References

A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition.
Guescini M et al, BMC Bioinformatics, 2008, 9: 326.

Examples

## Not run: 
data(guescini1)
ml <- modlist(guescini1, fct = l5())
pcrplot(ml[[1]])
sapply(2:84, function(x) pcrplot(ml[[x]], add = TRUE))
## End(Not run)

[Package qpcR version 1.1-8 Index]