reps2 {qpcR} | R Documentation |
A dilution experiment of two different cDNAs with five 4-fold dilutions of the cDNA, and three replicates for each dilution.
data(reps2)
A data frame with the PCR cycles and 30 qPCR runs with three replicates of five 4-fold dilutions. The replicates are defined by FX.Y.Z (X = cDNA number, Y = dilution number, Z = replicate number).
The real-time PCR was conducted with primers for the S27a housekeeping gene in a Lightcycler 1.0 instrument (Roche Diagnostics).
Heike Cappallo-Obermann, Bone Marrow Transplantation Unit, University Hospital Hamburg-Eppendorf.
### on single data data(reps2) m1 <- pcrfit(reps2, 1, 2, l3()) pcrplot(m1) ### on replicated data repData <- repform(reps2[,1:4], c(0,1,1,1)) m2 <- drmfit(values ~ Cycles, curve = Curve, data = repData, fct = l5()) pcrplot(m2)