batschetal {qpcR}R Documentation

qPCR dilution experiments with replicates (Lightcycler 1.0) from Batsch et al

Description

High quality 4-fold dilution experiments with 5 dilution steps and 3 replicates each. See 'Details' for the different setups.

Usage

batsch1
batsch2
batsch3
batsch4
batsch5

Format

Data frames with the PCR cycles and 15 qPCR runs with 3 replicates of five 4-fold dilutions. The replicates are defined by FX.Y (X = dilution number, Y = replicate number).

Details

The real-time PCR was conducted with a Lightcycler 1.0 instrument using the following setups:

batsch1: Primers for rat SLC6A14, Taqman probes
batsch2: Primers for human SLC22A13, Taqman probes
batsch3: Primers for pig EMT, Taqman probes
batsch4: Primers for chicken ETT, SybrGreen
batsch5: Primers for human GAPDH, SybrGreen

Source

Additional File 5 to the paper.

References

Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence.
Batsch A et al., BMC Bioinformatics, 2008, 9: 95.

Examples

data(batsch1)
ml <- modlist(batsch1, model = l4)
plot(ml)    

[Package qpcR version 1.2-4 Index]