midpoint {qpcR}R Documentation

Calculation of the exponential region midpoint according to Peirson et al

Description

Calculates the exponential region midpoint using the algorithm described under 'References'.

Usage

midpoint(object, noise.cyc = 1:5)

Arguments

object a fitted object.
noise.cyc the cycles defining the background noise.

Details

The 'midpoint' region is calculated by

F_{noise} times sqrt{frac{F_{max}}{F_{noise}}}

with Fnoise = the standard deviation of the background cycles and Fmax = the maximal fluorescence.

Value

A list with the following components:

f.mp the 'midpoint' fluorescence.
cyc.mp the 'midpoint' cycle, as predicted from f.mp.

Author(s)

Andrej-Nikolai Spiess

References

Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Peirson et al., Nucleic Acids Research, 2003, e73.

Examples

m1 <- pcrfit(reps, 1, 2, l5)
mp <- midpoint(m1) 
plot(m1)
abline(h = mp$f.mp, col = 2)
abline(v = mp$mp, col = 2)  

[Package qpcR version 1.2-4 Index]